Thermo Fisher Scientific DKK3 Monoclonal Antibody (4G7)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA5-15601 targets DKK3 in indirect ELISA, FACS, and WB applications, showing reactivity with human and non-human primate samples.
The immunogen is a purified recombinant fragment of human DKK3 expressed in E. coli.
MA5-15601 detects DKK3, which has a predicted molecular weight of approximately 38 kDa.

Target Information

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and two conserved cysteine-rich domains separated by a linker region, each containing 10 cysteine residues.
The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane.
The linker region contains 50–55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids.
All DKKs have several potential sites for cleavage by furin-type proteases.
Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord.
In situ hybridization reveals highest expression in mouse brain, eye, and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.