Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Ultra Violet 615, eBioscience

Applications

• Flow Cytometry (Flow): Tested dilution 5 µL (1.0 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 506, FITC
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule associated with integrin beta2 (CD18). CD11b is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It acts as a receptor for CD54, CD102, CD50, and iC3b, playing key roles in cell-cell and cell-matrix interactions.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry of human peripheral blood cells
• Recommended usage: 5 µL (1.0 µg) per test in a 100 µL final volume (10⁵–10⁸ cells/test)

Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is designed for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer or Brilliant Stain Buffer™ to minimize non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution.

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD11b (Integrin alpha-M, ITGAM, Integrin alpha-X, ITGAX)

• 165 kDa adhesion molecule forming a heterodimer with CD18 (Integrin beta-2).
• Also known as Mac-1 and CR3 (Complement receptor 3).
• Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.
• Functions as receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
• Commonly used as a microglial marker in nervous tissue.
• Expression increases during monocyte maturation and varies among macrophage types.
• Associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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