Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 615, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): Tested at ≤ 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-30S, 660-40S, 690
• NovaFluor Yellow 570
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

Description:
The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. It is expressed by dendritic cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion. N418 binds to CD11c on splenic dendritic cells and precipitates a 150/90 kDa heterodimer.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Use ≤ 0.5 µg per test (100 µL final volume). Optimize titration for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is suitable for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).

Excitation / Emission / Laser:
350 nm / 615 nm / Ultraviolet Laser

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD11, together with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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