Thermo Fisher Scientific F(ab`)2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, FITC

Applications

Tested Dilution

Immunocytochemistry (ICC/IF)

2.5 µg/mL

Flow Cytometry (Flow)

1:200-1:400

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Rat IgG

Conjugate

FITC FITC FITC

View additional formats

• [AP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Biotin/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/HRP/host/Rabbit/)
• [Texas Red](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Texas Red/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4°C or -20°C if preferred, store in dark

Shipping conditions

Wet ice

RRID

AB_2550664

Target

IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

F(ab`)2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.

For FACS analysis, use 50 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.