Thermo Fisher Scientific Legumain Polyclonal Antibody
Legumain 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 합성 펩타이드 면역원으로 제작되었으며, -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 100–150 on human Legumain protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
This gene encodes a cysteine protease that specifically hydrolyzes asparaginyl bonds. It may play a role in processing bacterial peptides and endogenous proteins for MHC class II presentation in lysosomal/endosomal systems. Activation occurs under acidic pH and is autocatalytic. Expression increases when monocytes differentiate into dendritic cells, and a fully active enzyme is produced after lipopolysaccharide stimulation. Overexpression is associated with many solid tumor types. A pseudogene exists on chromosome 13, and multiple alternatively spliced variants have been reported, with two validated forms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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