Thermo Fisher Scientific Phospho-ERK1/ERK2 (Tyr204) Polyclonal Antibody
ERK1/ERK2의 인산화 상태(Tyr204)를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot에 적합하며, 세포 신호전달 연구에 활용 가능. NIH-3T3 세포(PDGF-BB 처리)를 양성 대조군으로 추천. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphorylated peptide (KLH coupled) corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (Erk1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_1086439 |
Product Specific Information
A suggested positive control for this product is NIH-3T3 cell treated with PDGF-BB.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric G protein-coupled receptors, and transforming agents, activate the ERK1 and ERK2 pathways.
When growth factors bind to receptor tyrosine kinases, Ras interacts with Raf, a serine/threonine protein kinase, and activates it. Activated Raf phosphorylates serine residues in MEK1/2, which in turn phosphorylates tyrosine/threonine residues in extracellular-signal regulated kinase (ERK) 1/2.
Upon activation, ERKs phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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