Thermo Fisher Scientific VEGF-165 Polyclonal Antibody
Human VEGF-165를 인식하는 Rabbit Polyclonal Antibody로, WB 및 ELISA에 적합. 정제된 항체로 native 및 recombinant VEGF-165 검출 가능. 장기 보관 시 -20°C에서 안정적이며, 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human VEGF-165 protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | No preservative |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to immediate use.
- This purified antibody has been heated to 56°C for 30 minutes.
- Recognizes both native and recombinant human VEGF-165 in cell supernatants and certain body fluids.
- A control of similarly diluted normal rabbit IgG is recommended.
Target Information
The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF.
VEGF and its receptor system are key regulators of angiogenesis signaling.
Most tumors require angiogenesis, and VEGF is the most potent angiogenic cytokine linked to this process.
Five isoforms of VEGF are generated by alternative splicing from a single VEGF gene.
These isoforms bind to tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), expressed mainly on endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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