Thermo Fisher Scientific CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), PE, eBioscience

Thermo Fisher Scientific CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), PE, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: View 11 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse.
Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in naive mice (<0.5% of splenocytes) and is upregulated following IFN stimulation.
PDCA-1 cycles between cell surface and intracellular compartments and may regulate cytokine trafficking. It is the same protein recognized by antibody 120G8.

The eBio927 antibody shows functional activity; its epitope differs from eBio129c, enabling costaining and purification of pDCs.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on SJL splenocytes; ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration per assay.

Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein involved in B-cell development and growth.
BST2 expression supports stromal cell-dependent growth of pre-B-cell lines and is found in B-cells, T-cells, monocytes, NK cells, dendritic cells, and myeloma cells.
It functions as an inhibitor of retrovirus release from human cells, antagonized by HIV-1 Vpu. BST2 retention of virions may represent a therapeutic target for HIV/AIDS.
BST2 dysfunction is also associated with stomatitis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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