Thermo Fisher Scientific SNAP25 Monoclonal Antibody (OTI4B5), TrueMAB
SNAP25 단백질을 인식하는 mouse monoclonal 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 적합합니다. 인간 시료 반응성이 있으며, lyophilized 형태로 제공되어 안정적 보관이 가능합니다. 신경전달 조절 연구에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4B5 |
| Immunogen | Full length human recombinant protein of human SNAP25 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882)
Target Information
SNAP25 is a t-SNARE protein involved in the molecular regulation of neurotransmitter release.
It plays a role in synaptic function and associates with proteins responsible for vesicle docking and membrane fusion.
Additionally, SNAP25 regulates plasma membrane recycling via interaction with CENPF.
Mutations in the SNAP25 gene can result in myasthenic syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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