Thermo Fisher Scientific Phospho-PFKFB2 (Ser466) Polyclonal Antibody

Thermo Fisher Scientific Phospho-PFKFB2 (Ser466) Polyclonal Antibody

Applications and Tested Dilutions

• Western Blot (WB): 1:1,000–1:3,000
• Immunohistochemistry (Paraffin) (IHC-P): 1:50–1:200
• Immunocytochemistry (ICC/IF): 1:100–1:500

Product Specifications

Product Specific Information

This antibody detects endogenous levels of PFKFB2 only when phosphorylated at Ser466.

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. Through this mechanism, protein kinases mediate most signal transduction in eukaryotic cells, regulating metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.
The protein kinase family includes over 500 gene products and is classified into 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
The AGC kinase group consists of 63 kinases, including:

• Cyclic nucleotide-regulated protein kinase (PKA & PKG) family
• Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family
• Related to PKA and PKC (RAC/Akt) protein kinase family
• Kinases that phosphorylate G protein-coupled receptors (ARK)
• Kinases that phosphorylate ribosomal protein S6 (RSK)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.