Thermo Fisher Scientific PGK1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:20-1:1,000

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

in situ PLA (PLA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat, Yeast

Published species

Human, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 217 of Human PGK1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PGK1,uniProtId:P00558-1,ncbiNodeId:9606,antigenRange:1-217,antigenLength:417,antigenImageFileName:PA5-28612_PGK1_P00558-1_Rabbit.svg,antigenImageFileNamePDP:PA5-28612_PGK1_P00558-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.048 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546088

Product Specific Information

Recommended positive controls: 293T, PGK1 shRNA-transfected 293T, A431, HeLa, HepG2, mouse brain, Rat liver, Yeast wild type 303.

Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (87%), Xenopus laevis (84%), Pig (96%), Chicken (88%), Sheep (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Also known as ATP:3-phosphoglycerate 1-phosphotransferase showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.