Thermo Fisher Scientific PKC gamma Polyclonal Antibody
PKC gamma 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot 및 IHC(P/F)에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 뇌 및 척수 조직 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 1–314 of Human PKC gamma |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546094 |
Product Specific Information
- Recommended positive controls: 293T, mouse brain, rat brain
- Predicted reactivity: Mouse (98%), Rat (98%), Rabbit (99%), Bovine (98%)
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
Target Information
The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways.
PKC gamma is expressed solely in the brain and spinal cord, localized to neurons, and is required for neuronal functions such as long-term potentiation (LTP) and long-term depression (LTD). Knockout studies suggest involvement in neuropathic pain development. Defects in this protein are associated with spinocerebellar ataxia-14 (SCA14).
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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