Thermo Fisher Scientific Phospho-PLC gamma 1 (Tyr783) Polyclonal Antibody
Phospho-PLC gamma 1 (Tyr783) 폴리클로날 항체로, 인간·생쥐·랫트 시료에서 인산화된 PLCγ1 검출에 적합합니다. WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 사용 가능하며, Protein A로 정제된 고품질 항체입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human PLC gamma 1 around the phosphorylation site of Tyr783 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The mammalian Phospholipase Cγ (PLCγ) family includes two closely related proteins, PLCγ1 and PLCγ2. These isozymes share core structural domains and contain an additional PH domain, two SH2 domains, and one SH3 domain. Upon stimulation by extracellular signals such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCγ. Activated PLCγ catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), generating the second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG), which play key roles in intracellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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