Thermo Fisher Scientific AMPK alpha-1,2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 13-300 of human PRKAA1/PRKAA2 (NP_0062425) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AMPK alpha-1,2,uniProtId:Q13131-1,ncbiNodeId:9606,antigenRange:13-300,antigenLength:559,antigenImageFileName:PA5-116552_AMPK_alpha-1,2_Q13131-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116552_AMPK_alpha-1,2_Q13131-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.35 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901183

Product Specific Information

Positive Samples: HeLa, NIH/3T3, PC-12, Mouse brain, Rat heart

Immunogen sequence: AEKQKHDGRV KIGHYILGDT LGVGTFGKVK VGKHELTGHK VAVKILNRQK IRSLDVVGKI RREIQNLKLF RHPHIIKLYQ VISTPSDIFM VMEYVSGGEL FDYICKNGRL DEKESRRLFQ QILSGVDYCH RHMVVHRDLK PENVLLDAHM NAKIADFGLS NMMSDGEFLR TSCGSPNYAA PEVISGRLYA GPEVDIWSSG VILYALLCGT LPFDDDHVPT LFKKICDGIF YTPQYLNPSV ISLLKHMLQV DPMKRATIKD IREHEWFKQD LPKYLFPEDP SYSSTMID

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.