Norgen Herpes Simplex Virus 1 and 2 (HSV-1&2) TaqMan PCR Detection Kits
HSV-1 및 HSV-2 검출용 TaqMan PCR 키트로, 고감도·고특이적 프라이머/프로브와 PCR 컨트롤을 포함. 양성·음성 대조군 제공으로 실험 신뢰성 확보. 연구용으로 DNA 정제 후 신속한 바이러스 검출에 적합.
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Norgen Herpes Simplex Virus 1 and 2 (HSV-1&2) TaqMan PCR Detection Kits
SKU: TM31750
Features and Benefits
- Detection kits for HSV-1 & HSV-2
- Available in TaqMan format for analysis
Herpes Simplex Virus 1 and 2 (HSV-1&2) are both members of the Herpesviridae family and possess large double-stranded DNA genomes. HSVs are transmitted primarily via sexual contact, direct lesion contact, or perinatally. Most infections are asymptomatic, but lesions can appear on skin and mucous membranes of the mouth and genitals.
Primary HSV-1 infection may cause gingivostomatitis, eczema herpeticum, keratoconjunctivitis, and encephalitis. Secondary infections typically result in skin lesions near the nose, mouth, and genital regions.
TaqMan PCR Kit Options
HSV-1&2 TaqMan PCR Kit (100 reactions)
- Ready-to-use format including Master Mix and PCR control
- Specific Primer and Probe mix for target detection
- Positive and negative controls included
- Validates assay quality and checks PCR inhibition
HSV-1&2 TaqMan PCR Probe/Primer Set and Controls (100 reactions)
- Specific Primer/Probe mix and Positive Control for target pathogen
- Includes nuclease-free water
- Compatible with Norgen’s PCR Master Mix (#28007) or user-supplied mix
For research use only. Not intended for in vitro diagnostics.
Supporting Data
TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result.
Both PCR signals above the baseline from FAM and HEX channels indicate successful PCR amplification.
[그래프 1]
- Amplification (FAM channel)
- Y축: RFU / X축: Cycles
[그래프 2]
- Amplification (HEX channel)
- Y축: RFU / X축: Cycles
두 그래프 모두 “Amplification” 제목을 가지며, 첫 번째는 FAM channel (파란색), 두 번째는 HEX channel (초록색) 데이터를 나타냅니다.
TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result.
No target DNA detected in FAM channel; amplification in HEX channel confirms successful PCR.
상단 그래프 (FAM channel)
- Amplification
- Y축: RFU / X축: Cycles
하단 그래프 (HEX channel)
- Amplification
- Y축: RFU / X축: Cycles
두 그래프 모두 “Amplification” 제목을 가지며, 각각 FAM(파란색), HEX(초록색) 채널로 구분됩니다.
TaqMan PCR Kit - Figure 3. Example of PCR inhibition result.
No signal detected in either FAM or HEX channels, suggesting sample preparation should be repeated using recommended DNA purification kit.
상단 그래프 (FAM channel)
- Amplification
- Y축: RFU / X축: Cycles
하단 그래프 (HEX channel)
- Amplification
- Y축: RFU / X축: Cycles
공통 축: X축 Cycles (0~40), Y축 RFU (-100~900).
두 그래프 모두 동일한 형식의 증폭 곡선을 나타냅니다.
Storage Conditions and Product Stability
- Store all components at -20°C upon arrival
- Avoid repeated freeze-thaw (>2×) of Master Mix and Positive Control
- Stable for 2 years from production date without performance loss
- For intermittent use, aliquot reagents before freezing
Kit Components
| Component | Cat. TM31750 (100 preps) | Cat. TM31710 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 × 700 μL | - |
| HSV-1&2 Primer & Probe Mix | 280 μL | 280 μL |
| HSV-1&2 Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Documentation
Protocols
- (TM31750) HSV-1&2 TaqMan PCR Kit - Protocol (100 rxns)
- (TM31710) HSV-1&2 TaqMan Probe/Primer and Control Set - Protocol (100 rxns)
Safety Data Sheets
Related Products
- Herpes Simplex Virus 1 and 2 (HSV-1&2) TaqMan Probe/Primer and Control Set
- Urine DNA Isolation Kit (Slurry Format)
- Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit
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