Thermo Fisher Scientific CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), eFluor 450, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
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Product Specifications

Product Specific Information

Description:
The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in naive mice (<0.5% of splenocytes) and is upregulated upon IFN stimulation. It cycles between cell surface and intracellular compartments and may regulate cytokine trafficking. PDCA-1 (BST2) is the protein recognized by antibody 120G8.

The eBio927 antibody exhibits functional activity. Its epitope is distinct from eBio129c, allowing costaining, purification, and identification of pDCs.

Applications Reported: Flow cytometric analysis.
Applications Tested: Validated for flow cytometric analysis of mouse splenocytes. Use ≤0.5 µg per test (100 µL final volume). Optimize antibody titration for best results.

Fluorochrome Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility.

• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein involved in B-cell development and growth. Its expression supports stromal cell-dependent growth of pre-B-cell lines. BST2 is expressed in B-cell lineage, T-cells, monocytes, NK cells, dendritic cells, and myeloma cells. It also inhibits retrovirus release from human cells, antagonized by HIV-1 Vpu protein. BST2 depletion removes the need for Vpu in virus release, suggesting potential therapeutic relevance in HIV/AIDS. BST2 dysfunction is also associated with stomatitis.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

Additional Formats Available

• Alexa Fluor 488
• APC
• Biotin
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

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