Thermo Fisher Scientific Nucleoside Diphosphate Kinase A/nm23-H1 Monoclonal Antibody (NME1/2737)
인간 NME1 단백질을 인식하는 단클론 항체로, Western blot, IHC, ELISA 등 다양한 분석에 사용 가능. 단백질 A/G로 정제된 액상 형태이며 200 µg/mL 농도. 세포주 HeLa, A549, Jurkat에서 양성 대조로 활용 가능. 세포질 및 핵에서 발현, 4°C 보관.
- 카탈로그번호
- 4830-MSM7-P1
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Nucleoside Diphosphate Kinase A/nm23-H1 Monoclonal Antibody (NME1/2737)
Thermo Fisher Scientific Nucleoside Diphosphate Kinase A/nm23-H1 Monoclonal Antibody (NME1/2737)
Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | |
| ELISA | Assay-dependent | |
| Peptide Array | Assay-dependent | |
| Immunoelectrophoresis (IE) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NME1/2737 |
| Immunogen | Recombinant full-length human NME1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Stability: Antibody is stable for 24 months.
- Positive Control: HeLa, A549, or Jurkat cells.
- Expression: Ubiquitously expressed in all tissues.
- Cellular Localization: Cytoplasm and nucleus; cell-cycle dependent nuclear localization.
Specificity Comments:
The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines with varying metastatic capacities. Highly metastatic sub-lines show lower nm23 levels than less metastatic cells.
Based on sequence analysis, nm23 is closely related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B correspond to nm23-H1 and nm23-H2, respectively. nm23-H2 is identical to PuF, a transcription factor binding to nuclease hypersensitive elements within the c-Myc promoter.
Target Information
NME1 was identified due to reduced mRNA transcript levels in highly metastatic cells.
Nucleoside diphosphate kinase (NDK) forms a hexamer composed of A (encoded by NME1) and B (encoded by NME2) isoforms.
Mutations in NME1 are associated with aggressive neuroblastomas.
Two transcript variants encode different isoforms.
Co-transcription of NME1 and neighboring NME2 generates naturally occurring transcripts (NME1-NME2) encoding a fusion protein with sequences from both gene products.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.