Thermo Fisher Scientific Nucleoside Diphosphate Kinase A/nm23-H1 Monoclonal Antibody (NME1/2738)
NME1/nm23-H1 단백질을 인식하는 마우스 단일클론 항체로, Western blot, IHC, ELISA 등에 사용 가능. 인간 시료 반응성, 비결합형 액상 형태, 200 µg/mL 농도. 단백질 A/G 정제, 4°C 보관, 24개월 안정성.
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Thermo Fisher Scientific Nucleoside Diphosphate Kinase A/nm23-H1 Monoclonal Antibody (NME1/2738)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| ELISA | Assay-dependent |
| Peptide Array | Assay-dependent |
| Immunoelectrophoresis (IE) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NME1/2738 |
| Immunogen | Recombinant full-length human NME1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Stability: Antibody is stable for 24 months.
- Positive Control: HeLa, A549, or Jurkat cells.
- Expression: Ubiquitously expressed in all tissues.
- Cellular Localization: Cytoplasm and nucleus; cell-cycle dependent nuclear localization.
Specificity Comments:
The nm23 gene, a potential suppressor of metastasis, was identified by differential hybridization between murine melanoma sub-lines with varying metastatic capacities. Highly metastatic cells show lower nm23 expression. Sequence analysis shows nm23 is related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B correspond to nm23-H1 and nm23-H2, respectively. nm23-H2 is identical to PuF, a transcription factor binding to the human c-Myc promoter region.
Target Information
NME1 was discovered due to reduced mRNA levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) forms a hexamer composed of A (NME1) and B (NME2) isoforms. Mutations in NME1 are linked to aggressive neuroblastomas. Two transcript variants encode distinct isoforms. Co-transcription with the downstream NME2 gene produces fusion transcripts (NME1-NME2) encoding a hybrid protein sharing sequences from both genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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