
Thermo Fisher Scientific Nucleoside Diphosphate Kinase A/nm23-H1 Monoclonal Antibody (NME1/2738)
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Applications
Tested Dilution
Publications
Western Blot (WB)
1-2 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
1-2 µg/mL
ELISA (ELISA)
Assay-dependent
Peptide Array (Array)
Assay-dependent
Immunoelectrophoresis (IE)
Assay-dependent
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse / IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
NME1/2738
Immunogen
Recombinant full-length human NME1 protein if (typeof window.$mangular === undefined
|| !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{
targetFamily:
NME1,
uniProtId:
P15531-1,
ncbiNodeId:
9606,
antigenRange:
1-152,
antigenLength:
152,
antigenImageFileName:
4830-MSM8-P1_NME1_P15531-1_House_mouse.svg,
antigenImageFileNamePDP:
4830-MSM8-P1_NME1_P15531-1_House_mouse_PDP.jpeg,
sortOrder:
1}\]
; $mangular.isB2BCMGT = false
; $mangular.isEpitopesModalImageMultiSizeEnabled = true
;
View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}
Conjugate
Unconjugated Unconjugated Unconjugated
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A/G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Antibody is stable for 24 months.
Positive Control: HeLa, A549 or Jurkat cells. Ubiquitously expressed in all tissues. Cellular Localization: Cytoplasm. Nucleus. Cell-cycle dependent nuclear localization.
Specificity Comments: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
Target Information
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of A
(encoded by this gene) and B
(encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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