Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 7 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Biotin
• Brilliant UV 805
• eFluor 450
• eFluor 660
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein expressed on resting conventional B cells. Expression is modulated upon B-cell activation, while B-1 lineage (CD5+ B cells) do not express CD23. Soluble CD23 forms are biologically active and act as low affinity receptors for IgE, involved in regulation of IgE response and B-cell activation. CD21 is known to bind CD23.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimization: Antibody titration recommended for best performance

Fluorochrome Information:
Super Bright 436 is excited with a 405 nm violet laser and emits at 436 nm. Recommended detection using a 450/50 bandpass filter or equivalent. Ensure instrument compatibility.

Staining Recommendation:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD23 is a 45 kDa glycoprotein expressed on a subset of peripheral blood and tonsil B cells, and strongly expressed on EBV-transformed B lymphoblasts. It is identical to the low-affinity IgE receptor on B cells. CD23 expression is detected in neoplastic cells from B-cell chronic lymphocytic leukemia and certain lymphomas. Functionally, CD23 participates in B-cell growth, differentiation, and IgE production. Its soluble form acts as a potent mitogenic factor.
Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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