Thermo Fisher Scientific Phospho-MEK1/2 (Ser222, Ser226) Monoclonal Antibody (17C9.E1.H2.D7.H7.D5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

ELISA (ELISA)

1:40,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, lambda

Class

Monoclonal

Type

Antibody

Clone

17C9.E1.H2.D7.H7.D5

Immunogen

Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues surrounding the S222-226 phosphorylation site conjugated to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Specifically binds to the human MEK1 pS222 and MEK2 pS226 phosphorylated sites. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen.

Target Information

MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is known to be involved in the signaling during stress activate response, apoptosis and proliferative induction by cytokines. Mutations in the gene can lead to cardiofaciocutaneous syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.