Thermo Fisher Scientific IdU Monoclonal Antibody (32D8.D9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:5,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

1:1,000-1:10,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

32D8.D9

Immunogen

Anti-IdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for IdU. Cross-reactivity is not observed with BrdU, CldU or FdU.

Target Information

Idu (Idoxuridine) is a nucleoside analogue, a modified form of deoxyuridine, similar enough to be incorporated into viral DNA replication, but the iodine atom added to the uracil component blocks base pairing. It is used only topically due to cardiotoxicity. It was synthesized by William Prusoff in the late 1950s.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.