Thermo Fisher Scientific PKC alpha Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Recommended positive controls: Jurkat, Mouse brain
• Predicted reactivity: Mouse (100%), Zebrafish (90%), Xenopus laevis (94%), Rabbit (100%), Chicken (98%), Rhesus Monkey (100%), Bovine (99%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.

PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate various protein targets and are involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters.

PKC alpha plays roles in cell adhesion, transformation, cycle checkpoint, and volume control. Knockout studies in mice suggest it may regulate cardiac contractility and Ca²⁺ handling in myocytes.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information: www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 없음)