Thermo Fisher Scientific IL-3 Polyclonal Antibody
Rat IL-3에 특이적인 Thermo Fisher Scientific의 Goat Polyclonal Antibody로, WB, ICC/IF, Neutralization 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결 건조 형태로 제공. 연구용으로만 사용.
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Thermo Fisher Scientific IL-3 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| Neutralization (Neu) | 0.75–3 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant rat IL-3 (Ile27–Cys169) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609732 |
Product Specific Information
- In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse IL-3 and less than 1% cross-reactivity with recombinant human IL-3 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of antibody by the LAL method.
Target Information
Interleukin 3 (IL-3), also known as Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils.
IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Its functions are enhanced or dependent on co-stimulation with other cytokines.
Human and mouse IL-3 share only 29% sequence identity at the amino acid level, and bioactivity is highly species-specific.
IL-3 supports proliferation and differentiation of hematopoietic cells and has been shown to possess neurotrophic activity, potentially associated with neurologic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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