Thermo Fisher Scientific GSK3 alpha/beta Monoclonal Antibody (21A)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 47 publications 47 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

1:1,000

-

Immunoprecipitation (IP)

5-10 µg

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat, Xenopus

Published species

Human, Mouse, Non-human primate, Rat, Shrew

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

21A

Immunogen

The antibody was produced in mouse against a recombinant Xenopus laevis protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2

Contains

0.01% sodium azide

Storage conditions

4°C short term, -80°C long term

RRID

AB_2533693

Product Specific Information

44-610 is an antibody that`s produced in mouse against a recombinant Xenopus laevis protein. |Has been successfully used ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blot procedures.

Target Information

Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimers disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.