
Thermo Fisher Scientific Phospho-c-Raf (Ser259) Polyclonal Antibody
Phospho-c-Raf (Ser259) 인산화 부위를 인식하는 rabbit polyclonal antibody. Western blot, ICC/IF, IP에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 제품이며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Antiserum produced against a chemically synthesized phosphopeptide derived from a region of human c-Raf that contains serine 259. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco's PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533669 |
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates the dual specificity protein kinases MEK1 and MEK2, which in turn activate ERK1 and ERK2. These activated ERKs regulate gene expression involved in cell cycle control, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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