Thermo Fisher Scientific Transferrin Receptor Monoclonal Antibody (MEM-189)
인간 Transferrin Receptor(CD71)을 인식하는 Mouse monoclonal antibody (Clone MEM-189). Western blot, ICC/IF, Flow cytometry, Immunoprecipitation에 사용 가능. Protein A 정제, 1 mg/mL 농도, 4°C 보관. 연구용으로만 사용.
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Applications 및 권장 희석 배수
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | Assay-dependent | 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-189 |
| Immunogen | Tissue / cell preparation (Human, KG1 acute myelogenous leukemia cell line). Target: Transferrin Receptor (CD71), UniProt ID: P02786-1, Range: 1–760 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_558277 |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor.
Transferrin receptor is necessary for the development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from conditions such as rheumatoid arthritis, pregnancy, irritable bowel syndrome, and HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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