Thermo Fisher Scientific Basiliximab Chimeric Recombinant Human Monoclonal Antibody (Basiliximab)
Basiliximab은 인간 IL-2 수용체 α-사슬(CD25)에 특이적으로 결합하는 키메라형 재조합 단일클론 항체입니다. T세포 활성화를 억제하여 면역 반응을 조절하며, 다양한 면역학적 연구에 활용됩니다. Western blot, IHC, ICC, Flow cytometry 등에 적용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 5 µg/mL | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Flow Cytometry (Flow) | Assay-dependent | |
| Inhibition Assays (IA) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Cynomolgus monkey, Human, Rhesus monkey |
| Host / Isotype | Human / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Basiliximab |
| Immunogen | Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074281 |
Product Specific Information
Basiliximab recognizes the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit. Binding is abolished when two or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R complex (alpha-beta-gamma-c), and T cells express CD25 only upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
Target Information
Basiliximab is a recombinant chimeric (murine/human) monoclonal antibody (IgG1κ) functioning as an immunosuppressive agent. It specifically binds to and blocks the interleukin-2 receptor α-chain (IL-2R alpha, CD25 antigen) on activated T-lymphocytes.
This 144 kDa glycoprotein is produced from a genetically engineered mouse myeloma cell line expressing plasmids with human heavy and light chain constant region genes and mouse variable region genes encoding the RFT5 antibody.
Basiliximab binds with high affinity to the α-subunit (CD25) of the high-affinity IL-2 receptor, inhibiting IL-2 binding and subsequent T-cell activation, thereby preventing immune response against transplanted organs such as kidneys.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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