Thermo Fisher Scientific VPS35 Monoclonal Antibody (10A8), PerCP
VPS35 단백질을 검출하기 위한 PerCP 형광 표지 단클론 항체. 인간, 생쥐, 랫트 시료에 반응하며 WB, IHC, ICC, IP 등 다양한 응용 가능. 고순도 Protein G 정제, 4°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific VPS35 Monoclonal Antibody (10A8), PerCP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10A8 |
| Immunogen | Full length recombinant human VPS35 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932401 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-45947 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. Detects approximately 92 kDa.
Target Information
VPS35 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of the retromer complex, which mediates retrograde transport of proteins from endosomes to the trans-Golgi network. Structural similarity between yeast and human retromer proteins suggests functional conservation. VPS35 serves as the core of the retromer complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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