Thermo Fisher Scientific VPS35 Monoclonal Antibody (10A8), FITC
VPS35 단백질 검출용 FITC 결합 단클론 항체로 Western blot, IHC, ICC/IF, IP에 사용 가능. 사람, 생쥐, 랫트 반응성. 고순도 Protein G 정제, 1 mg/mL 농도. 4°C 암소 보관, 형광 분석에 적합.
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Thermo Fisher Scientific VPS35 Monoclonal Antibody (10A8), FITC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10A8 |
| Immunogen | Full length recombinant human VPS35 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932400 |
Additional Formats
- APC (Cat. No. MA5-45945)
- PerCP (Cat. No. MA5-45947)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45946 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Detects approximately 92 kDa.
Target Information
VPS35 is part of the vacuolar protein sorting (VPS) gene family. The encoded protein is a component of the retromer complex involved in retrograde transport of proteins from endosomes to the trans-Golgi network. Structural similarity between yeast and human proteins suggests functional conservation. Expression studies indicate that this protein interacts directly with VPS35, serving as the core of the retromer complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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