Thermo Fisher Scientific Pyruvate Carboxylase Polyclonal Antibody
Thermo Fisher Scientific의 Pyruvate Carboxylase Polyclonal Antibody는 인간 및 랫트 반응성을 가지며 다양한 응용(WB, IHC, ICC, IP)에 적합합니다. 항원 친화 크로마토그래피로 정제된 비결합 액상 항체로, 장기 보관 시 -20°C에서 안정적입니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Pyruvate Carboxylase Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
| Immunoprecipitation (IP) | 1:100–1:900 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Pyruvate Carboxylase. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.69 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736487 |
Product Specific Information
- Positive Control: 293T, A431, HeLa, HepG2
- Predicted Reactivity: Pig (97%), Chicken (84%), Rhesus Monkey (100%), Bovine (96%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron located exclusively in the mitochondrial matrix. It is involved in gluconeogenesis, lipogenesis, insulin secretion, and synthesis of the neurotransmitter glutamate. Mutations in this gene are associated with pyruvate carboxylase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: PA5-78100_Pyruvate_Carboxylase_P11498-1_Rabbit.svg, PA5-78100_Pyruvate_Carboxylase_P11498-1_Rabbit_PDP.jpeg)
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