Thermo Fisher Scientific CD28 Recombinant Mouse Monoclonal Antibody (JJ316)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

T-Cell Activation (TCA)

Assay-dependent

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ316

Immunogen

Made by immunizing BALB/c mice with rat CD28 cDNA-transfected A20/J cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074313

Product Specific Information

Specificity: Recognises rat CD28 and can trigger T-cell proliferation in vitro and in vivo.

Target Information

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary`s Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.