Norgen 2X PCR Master Mix
PCR 반응에 필요한 모든 구성요소가 포함된 2X 농도의 ready-to-use 마스터믹스로, 높은 감도와 수율을 제공하며 오염 위험을 최소화합니다. 안정성이 뛰어나며 반복적인 동결-해동에도 성능을 유지합니다. -20℃ 보관 시 1년 이상 안정적입니다.
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Norgen 2X PCR Master Mix
SKU: 28007
A ready-to-use solution optimized for robust amplification of high yield products.
Features and Benefits
- Convenient ready-to-use solution
- High sensitivity and yield
- Robust amplification
Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl₂, KCl and a PCR enhancer/stabilizer.
The user needs only to add template, primer set, and water to the master mix to set up the PCR reaction.
This convenient 2X PCR Master Mix reduces setup time and contamination risk, especially for large numbers of reactions.
The optimized mix allows robust amplification of DNA templates with high yields of PCR products.
Taq DNA Polymerase is a highly thermostable enzyme with 5´→3´ polymerase activity and minimal 5´→3´ exonuclease activity.
The Taq source is an E. coli strain containing a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Product Details
| 항목 | 내용 |
|---|---|
| SKU | 28007 |
| 제품명 | 2X PCR Master Mix |
| 형태 | Ready-to-use solution |
| 구성 성분 | Taq DNA Polymerase, dNTPs, Reaction Buffer, MgCl₂, KCl, PCR Enhancer/Stabilizer |
| 반응 수 | 100 × 20 µL reactions |
| 보관 조건 | -20℃ (일상 사용 시 4℃에서 최대 3개월) |
| 안정성 | 다중 동결-해동 후에도 안정적, 1년 이상 보관 가능 |
Supporting Data
Figure 1. Successful Amplification of Different Sized Amplicons
Norgen’s 2X PCR Master Mix was used to amplify various amplicon sizes from the adenovirus E2 region.
Lanes 1–2: 189 bp, Lanes 3–4: 565 bp, Lanes 5–6: 1501 bp, Lanes 7–8: 2202 bp.
Lane M1: PCR Sizer 100 bp DNA Ladder (Cat #11400), Lane M2: MidRanger 1kb DNA Ladder (Cat #11700).
All products were amplified with high sensitivity and yield.
이미지 내 텍스트 (OCR 추출):
| M1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | M2 |
|---|
Figure 2. Highly Stable after Multiple Freeze-Thaw Cycles
The stability of Norgen’s 2X PCR Master Mix was tested through multiple freeze-thaw cycles, then used to amplify 16s rDNA (1.5 kb) of Staphylococcus aureus gDNA.
The mix showed excellent robustness and stability at -20ºC.
M: FastRunner DNA Ladder (Cat#12800).
10 μL of each PCR reaction was loaded on a 1% TAE agarose gel.
이미지 내 텍스트 (OCR 추출):
Cycles 2 3 4 5 10 15 20 25
← 1.5 kbReagents Supplied (Cat #28007)
- 2X PCR Master Mix (1 Vial, 100 Reactions)
Storage Conditions and Product Stability
- Store at -20ºC
- For everyday use, aliquot can be stored at 4ºC for up to 3 months
- Stable for multiple freeze-thaw cycles
- Shelf life: ≥1 year under proper storage
Documentation
Citations
| Title | Citation | Authors |
|---|---|---|
| An Assessment of Water Quality Index and Some Enteric Bacteria Characteristics of Ossiomo River, Edo State, Nigeria | Cognizance Journal 2023 | Augustine Brian ODIGIE; Sunday Eghosasere OMOZUWA |
| Risk Assessment of Heavy Metals on Human Health and Bacteriological Qualities of Ossiomo River, Edo State, Nigeria | Arid Zone Journal of Basic and Applied Research 2023 | Augustine B. Odigie; Sunday Eghosasere Omozuwa |
| Strategy for immunological analysis of pro-inflammatory cytokine marker studies with chronic hepatitis B virus in Southwestern region of Saudi Arabia | Journal of Infection and Public Health 2023 | Amal F. Alshammary; Raed Farzan; Sarah F. Alsobaie; Arwa A. Alageel; Imran Ali Khan |
| Dose-response mutation and spectrum analyses reveal similar responses at two microsatellite loci in benzo(a)pyrene-exposed mouse spermatogonia | Mutagenesis 2017 | Rowan-Carroll, A.; Beal, M. A.; Williams, A.; Marchetti, F.; Yauk, C. L. |
| Amendment of amino acid in Q192R genetic polymorphism of paraoxonase 1 is a conventional risk factor for type 2 diabetes mellitus in the Saudi population | Int J Clin Exp Med 2016 | Alharbi, K. K.; Alharbi, F. K.; Ghneim, H. K.; Al-Sulaiman, A. M.; Alodhayani, A. A.; Khan, I. A |
| Production of Lipases in Solid-State Fermentation by Aspergillus niger F7-92 with Agricultural Residues | Journal of Microbiology, Biotechnology and Food Sciences 2015 | OO Adio; SO Kareem; MB Osho; AM Omemu |
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