Thermo Fisher Scientific CD134 (OX40) Monoclonal Antibody (OX-86), PE, eBioscience
Mouse CD134 (OX40) 단백질을 인식하는 OX-86 클론의 rat IgG1, kappa 형 단일클론 항체로 PE 형광표지되어 있습니다. Flow cytometry에 최적화되어 있으며 활성화된 T 세포 분석에 적합합니다. 565/576 nm에서 여기/방출, 4°C 암소 저장.
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Thermo Fisher Scientific CD134 (OX40) Monoclonal Antibody (OX-86), PE, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 13 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OX-86 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465853 |
Additional Formats Available
- Unconjugated
- APC
- Functional Grade
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 780
- Custom conjugation available upon request
Product Specific Information
The OX-86 monoclonal antibody reacts with mouse CD134 (OX40), a 50 kDa type I membrane glycoprotein of the TNF receptor superfamily expressed by activated mouse T lymphocytes.
Mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. Interaction with CD252 (OX40 ligand) is involved in T cell-dependent humoral responses, primary T cell expansion, survival, memory T cell pool regulation, and tolerance in CD4+ T cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on activated mouse splenocytes at ≤0.5 µg/test (100 µL final volume; 10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
OX40 is a receptor on T cells that promotes their proliferation and survival upon activation, enhancing immune responses against cancer and infections.
OX40 agonists are under investigation for cancer immunotherapy and may enhance the efficacy of chemotherapy or checkpoint inhibitors, with potential applications in autoimmune diseases and allergies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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