Thermo Fisher Scientific UNG Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These isoforms are generated by alternative splicing and two differentially regulated promoters (PA and PB). The cDNAs for UNG1 and UNG2 are similar in size (2061 and 2058 bp), and thus their mRNAs are not resolved as two species in gel electrophoresis.

Nuclear UNG2 differs from mitochondrial UNG1 by 44 amino acids at the N-terminal sequence, which is not required for catalytic activity. The UNG gene products play a major role in repairing mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues. Expression levels of UNG1 and UNG2 vary by cell and tissue type and are cell-cycle regulated. Generally, UNG expression and activity are higher in proliferating than in non-proliferating cells.

• UNG1: 304 amino acids
• UNG2: 313 amino acids

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.