Thermo Fisher Scientific UNG Polyclonal Antibody
Thermo Fisher Scientific의 UNG Polyclonal Antibody는 인간 UNG 단백질을 인식하는 토끼 다클론 항체로, Western blot 및 Immunoprecipitation에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 4°C에서 보관하십시오.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 25 and 50 of Human Uracil-DNA Glycosylase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These isoforms are generated by alternative splicing and the use of two differentially regulated promoters (PA and PB).
UNG1 and UNG2 cDNAs are similar in size (2061 and 2058 bp, respectively), resulting in unresolved mRNA species in gel electrophoresis.
Nuclear UNG2 differs from mitochondrial UNG1 by 44 amino acids in the N-terminal sequence, which is not essential for catalytic activity.
The UNG gene products play a key role in repairing mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues. Expression levels of UNG1 and UNG2 vary between cell and tissue types and are regulated by the cell cycle. Generally, UNG expression and activity are higher in proliferating cells compared to nonproliferating ones.
UNG1 is a 304 amino acid protein, while UNG2 is a 313 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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