Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Red 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 4 µL (0.6 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV, Violet, Yellow series
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

Description:
The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with the B-cell receptor (BCR). Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B-cell proliferation.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage is 4 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for best results.

Usage Recommendation:
Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer.

• Add 5 µL CellBlox per sample when staining with NovaFluor conjugates.
• For single-color controls, use 5 µL CellBlox per 100 µL of cell sample (10³–10⁸ cells).

Spectral Properties:

• Excitation: 637 nm
• Emission: 659 nm
• Laser: 633–640 nm (Red Laser)

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescence signatures and minimal spillover and spread.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and certain myeloid leukemia cells. CD19 serves as a signal-amplifying coreceptor for the B-cell receptor (BCR) and regulates B-cell proliferation. Mutations in CD19 can result in hypogammaglobulinemia, while overexpression leads to B-cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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