Thermo Fisher Scientific Carbonic Anhydrase VA/VB Monoclonal Antibody (487908)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

8-25 µg/mL

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

487908

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase VA/VB (CA5A/5B) Ala40-Ser305 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Carbonic Anhydrase VA/VB,uniProtId:P35218-1,ncbiNodeId:9606,antigenRange:40-305,antigenLength:305,antigenImageFileName:MA5-24199_Carbonic_Anhydrase_VAVB_P35218-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24199_Carbonic_Anhydrase_VAVB_P35218-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Carbonic Anhydrase VA/VB,uniProtId:Q9Y2D0-1,ncbiNodeId:9606,antigenRange:40-305,antigenLength:317,antigenImageFileName:MA5-24199_Carbonic_Anhydrase_VAVB_Q9Y2D0-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24199_Carbonic_Anhydrase_VAVB_Q9Y2D0-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608926

Product Specific Information

In Western blots, no cross-reactivity with recombinant human CA1, 4, or CA6-14 is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA5A gene encodes for the CA V protein which is expressed in the mitochondrial matrix in liver tissues, but can also be detected in many other tissues. In ureagenesis, the CA V protein may be involved in providing bicarbonate to carbamoyl phosphate synthetase. CA5B belongs a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. This protein is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. The protein has a wider tissue distribution than CA VA, which is restricted to the liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.