Thermo Fisher Scientific SERPINA12 Monoclonal Antibody (484223)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

484223

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A12 Leu20-Lys414 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SERPINA12,uniProtId:Q8IW75-1,ncbiNodeId:9606,antigenRange:20-414,antigenLength:414,antigenImageFileName:MA5-24197_SERPINA12_Q8IW75-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24197_SERPINA12_Q8IW75-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2606845

Product Specific Information

In Western blots, no cross-reactivity with recombinant human (rh) Serpin A1, A3, A4, A5, A6, B8, 9, C1, D1, E1, F2, G1, I1, I2, recombinant mouse (rm) Serpin E2, F1, recombinant rat Serpin B6, rhA1 Antitrypsin, rhAngiotensinogin, or rmPEDF is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Vaspin is a newly described adipocytokine expressed predominantly in visceral white adipose tissues. Structure analysis of vaspin predicts the presence of three beta-sheets, nine alpha-helices, and one central loop, which are distinctive structural features of Serpin family members. The serpins are irreversible (suicidal) serine-protease inhibitors, characterized by having more than 30% sequence homology with alpha1-antitrypsin and a conserved tertiary structure, which contains an exposed reactive center loop that acts as a pseudo-substrate for the target proteinase. Members of this family play an important role in a number of fundamental biological processes, including blood coagulation, fibrinolysis, complement activation, angiogenesis, inflammation, and tumor suppression. In humans, the serpins represent approximately 2% of total serum proteins, of which 70% is alpha1-antitrypsin. Vaspin exhibits 40.2% sequence identity with alpha1-antitrypsin. Yet, its protease inhibitory activity is still unknown. Vaspin mRNA expression in visceral fat is positively correlated with BMI and percent of body fat. Administration of vaspin to obese mice improved glucose tolerance and insulin sensitivity, reflected by normalized blood glucose levels. It also led to the reversal of altered expression of diabetes-relevant adipocytokines, including leptin, adiponectin, resistin, and TNF-alpha. These findings suggest a potential clinical use for Vaspin in ameliorating certain aberrations seen in the diabetic/obesity metabolic syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.