Thermo Fisher Scientific Gemin 3 Monoclonal Antibody (12H12)
Gemin3 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA, IP 등에 사용 가능. 1 mg/mL 농도의 액상 형태이며 Protein A로 정제됨. 인간, 생쥐, 햄스터, 랫트 시료에 반응하며 연구용으로 적합.
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Thermo Fisher Scientific Gemin 3 Monoclonal Antibody (12H12)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1 µg for 10^6 cells |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12H12 |
| Immunogen | Recombinant 6His-tag COOH-terminal domain of Gemin3 (amino acids 368–548) from SP2/0 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2092399 |
Product Specific Information
MA1-5779 detects Gemin3 in human samples.
It has been successfully used in Western blot, immunoprecipitation, immunofluorescence, and ELISA procedures.
MA1-5779 detects a band of approximately 105 kDa.
The immunogen is recombinant 6His-tag COOH-terminal domain of Gemin3.
Target Information
Gemin 3 is a member of the DEAD box protein family characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif and functions as an RNA helicase.
DEAD box proteins are involved in RNA secondary structure modification processes such as translation initiation, splicing, and ribosome/spliceosome assembly.
Gemin 3 participates in the SMN complex and may play a catalytic role in its function on RNPs.
Dysfunction of Gemin 3 is associated with spinal muscular dystrophy and muscular atrophy.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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