Thermo Fisher Scientific Gemin 2 Monoclonal Antibody (2E17)
Gemin2 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단일클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA, IP에 사용 가능. Human, Mouse, Xenopus 반응성. Protein A 정제, 1 mg/mL 농도, PBS 내 0.1% sodium azide 포함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1 µg for 10^6 cells |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Xenopus |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E17 |
| Immunogen | His-Gemin 2 fusion protein (amino acids 12–280, starting at 2nd methionine) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2187799 |
Product Specific Information
- MA1-5777 detects Gemin2 in human and Xenopus samples.
- Validated for Western blot, immunoprecipitation, immunofluorescence, and ELISA.
- Detects a ~34 kDa band.
- Immunogen: His-Gemin2 (starting at 2nd methionine).
- Store at 4°C for one month, or in small aliquots at -20°C for longer storage.
Target Information
SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and pre-mRNA splicing. SIP1 interacts directly with SMN and is required for formation of the SMN complex. Knockout studies in mice have shown disrupted snRNP assembly and motor neuron degeneration, suggesting SIP1 may have roles beyond the SMN complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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