Thermo Fisher Scientific Gemin 2 Monoclonal Antibody (2E17)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Flow Cytometry (Flow)

1 µg for 10^6 cells

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Xenopus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E17

Immunogen

His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Gemin 2,uniProtId:O14893-1,ncbiNodeId:9606,antigenRange:12-280,antigenLength:280,antigenImageFileName:MA1-5777_Gemin_2_O14893-1_House_mouse.svg,antigenImageFileNamePDP:MA1-5777_Gemin_2_O14893-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2187799

Product Specific Information

MA1-5777 detects Gemin2 in human and xenopus samples.

MA1-5777 has been successfully used in Western blot, immunoprecipitation, immunofluorescence and ELISA procedures. MA1-5777 detects a band of approximately 34 kDa.

The MA1-5777 immunogen is His-Gemin2 (starting at 2nd methionine).

Store at 4ºC for one month, or in small aliquots at -20ºC for longer periods.

Target Information

SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.