Thermo Fisher Scientific Arginase 1 (ARG1) Monoclonal Antibody (OTI4G3)

Applications

Tested Application

• Western Blot (WB): 1:500 dilution tested

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
• For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme that catalyzes the conversion of L-arginine to urea and L-ornithine, the final step of the urea cycle.
The resulting polyamines are essential for cell proliferation and detoxification of byproducts from protein degradation.
By degrading arginine, Arginase 1 reduces substrate availability for nitric oxide synthase, thereby down-regulating nitric oxide production.

• Expression: Found in liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells.
• Human: Expressed in blood neutrophils but not CCR3+ granulocytes.
• Mouse: Marker of alternatively activated macrophages (M2a).
• Pathological relevance: Commonly expressed in myeloid cells infiltrating tumors and in hepatocellular carcinoma.
• Clinical relevance: Defects in Arginase 1 cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.