Thermo Fisher Scientific Arginase 1 (ARG1) Monoclonal Antibody (OTI4D7)

Applications

Tested Application

• Western Blot (WB): 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882) to ensure carrier-free conditions.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme that converts L-arginine to urea and L-ornithine, completing the urea cycle.
It regulates nitric oxide production by limiting substrate availability for NO synthase.
Arginase 1 is expressed in the liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells in both humans and mice.
In mice, it is a hallmark of alternatively activated macrophages (M2a).
Arginase-1 expression is often observed in tumor-infiltrating myeloid cells and hepatocellular carcinomas.
Defects in the ARG1 gene cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.