Thermo Fisher Scientific CD3 Monoclonal Antibody (7D6), APC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

7D6

Immunogen

Human CD3 epsilon

Conjugate

APC APC APC

View additional formats

• Unconjugated
• Alexa Fluor 488
• APC-Alexa Fluor 750
• FITC
• PE
• PE-Alexa Fluor 610
• PE-Alexa Fluor 700
• PE-Cyanine5.5
• PE-Cyanine7
• PE-Texas Red
• TRI-COLOR
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with sucrose, BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373679

Product Specific Information

Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

Analyte Specific Reagent