
Thermo Fisher Scientific Goat anti-Human IgG Fab Superclonal Secondary Antibody, HRP
인간 IgG Fab를 특이적으로 인식하는 HRP 결합 염소 유래 Superclonal 재조합 2차 항체입니다. Fc 부위에 반응하지 않으며, 높은 특이성과 로트 간 일관성을 제공합니다. Western blot 및 ELISA에 최적화되어 있습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 |
| ELISA | 1:1,000–1:4,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Secondary Antibody |
| Clone | NA |
| Immunogen | Purified Human IgG |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with proprietary stabilizer |
| Contains | Proprietary preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2930933 |
| Target | IgG Fab |
| Antibody Form | Whole Antibody |
Additional Formats
- Alexa Fluor 488
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- Request custom conjugation available
Product Specific Information
This Goat anti-Human IgG Fab Superclonal™ Recombinant Secondary Antibody detects human IgG Fab with specificity to the light chains (kappa and lambda) and heavy chain CH1. It does not detect Fc and shows no cross-reactivity with immunoglobulins from other species.
Recombinant antibodies are produced using cloned genes encoding the desired antibody in an expression system, providing enhanced specificity, animal origin-free formulation, and lot-to-lot consistency.
Superclonal™ Recombinant antibodies are defined pools of recombinant monoclonal antibodies selected and combined to achieve complementary and optimal epitope coverage for improved performance.
Each lot’s sensitivity and specificity are validated by ELISA.
Careful titration is recommended for optimal assay performance.
Target Information
Anti-Human secondary antibodies are affinity-purified and specifically recognize human immunoglobulins. They are used for detection, sorting, or purification of human antibody targets. Secondary antibodies enhance assay versatility and sensitivity by enabling various detection systems (HRP, AP, fluorescence) and signal amplification.
Typically, they are produced by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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