Thermo Fisher Scientific Goat anti-Human IgG (Heavy chain) Superclonal Secondary Antibody
인간 IgG 헤비체인에 특이적으로 반응하는 염소 유래 Superclonal 재조합 2차 항체입니다. 높은 특이성과 로트 간 일관성을 제공하며, WB, ICC/IF, ELISA 등에 적합합니다. 비결합형 액상 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 |
| ELISA | 1:1,000–1:4,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Human IgG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2930917 |
| Target | IgG (Heavy chain) |
| Antibody Form | Whole Antibody |
Product Specific Information
This Goat anti-Human IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody specifically detects human IgG heavy chain with no cross-reactivity to light chain and other species immunoglobulins, except moderate cross-reactivity towards Monkey IgG.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, animal origin-free formulation, and lot-to-lot consistency.
Superclonal™ Recombinant antibodies are a defined pool of recombinant monoclonal antibodies, manufactured by selecting and pooling individual clones to provide complementary, specific, and optimal epitope coverage to achieve enhanced performance.
The sensitivity and specificity of each lot is confirmed using ELISA.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of the specified target. Secondary antibodies offer increased versatility, enabling use with many detection systems (e.g., HRP, AP, fluorescence). They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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