Thermo Fisher Scientific DNMT3A Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase.
• Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Suggested positive control: antigen standard for DNMT3A (transient overexpression lysate), HCT116 cell lysates.

Target Information

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with cancer development.
Three families of mammalian DNA methyltransferase genes have been identified: Dnmt1, Dnmt2, and Dnmt3.

• Dnmt1: Constitutively expressed in proliferating cells; its inactivation causes global demethylation and embryonic lethality.
• Dnmt2: Expressed at low levels in adult tissues; inactivation does not affect DNA methylation.
• Dnmt3a / Dnmt3b: Strongly expressed in ES cells; downregulated in differentiating ES cells and low in adult somatic tissue.
  Mutations in the Dnmt3b gene are associated with ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.