
Thermo Fisher Scientific Ubiquitin Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 사용 가능한 Rabbit Polyclonal 항유비퀴틴 항체. 사람, 마우스, 랫트 등 다양한 종에 반응하며, 고보존성 단백질 인식. 연구용으로 단백질 분해 경로 및 유비퀴틴 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:500–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:500–1:1,000 | View 1 publication |
| Immunoprecipitation (IP) | – | View 1 publication |
| in situ PLA (PLA) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Human, Mouse, Rat |
| Published Species | Human, Mouse, Yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified ubiquitin conjugated with glutaraldehyde to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 5 mM sodium azide |
| Storage Conditions | Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2241302 |
Target Information
Ubiquitin is a highly conserved protein that plays a major role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein.
This gene encodes a fusion protein consisting of ubiquitin at the N-terminus and ribosomal protein S27a at the C-terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S ribosomal subunit, belongs to the S27AE family, contains C4-type zinc finger domains, and is located in the cytoplasm.
Pseudogenes derived from this gene are present in the genome. Similarly, ribosomal proteins L40 and S30 are synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins. Multiple alternatively spliced transcript variants encoding the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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