Thermo Fisher Scientific H3K36me2 Monoclonal Antibody (T.571.7), ChIP-Verified
H3K36me2 단일클론 항체(T.571.7)는 ChIP 검증된 Rabbit IgG 항체로, 인간 및 설치류 시료에 반응합니다. 다양한 응용(ChIP, WB, IHC, ICC)에 적합하며, 고순도 친화 크로마토그래피 정제 제품입니다. -20°C에서 보관하며 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:800–1:1600 | - |
| ChIP assay (ChIP) | 1–5 µg | - |
| Peptide Array (Array) | 0.25 µg/mL | - |
| CUT&RUN (C&R) | – | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | T.571.7 |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys36 is di-methylated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 52 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10983670 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with di-methylated histone H3 Lys4, Lys9, Lys27, Lys79, or methylated histone H4 Lys20.
- Click here for Master Lot linking information
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histones, it forms the nucleosome structure that organizes DNA.
Histones play a crucial role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications such as methylation, acetylation, and phosphorylation on the N-terminal tail of histone H3 influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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