Thermo Fisher Scientific H3K36me2 Monoclonal Antibody (T.571.7), ChIP-Verified

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

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Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunocytochemistry (ICC/IF)

1:800-1:1600

-

ChIP assay (ChIP)

1-5 µg

-

Peptide Array (Array)

0.25 µg/mL

-

CUT&RUN (C&R)

-

- -

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

T.571.7

Immunogen

Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys36 is di-methylated

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

52 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10983670

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with di-methylated histone H3 Lys4, Lys9, Lys27, Lys79 or methylated histone H4 Lys20.

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Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.