Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is자세히 알아보기

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis. The kit can be used to synthesize cDNA from for a wide range of input RNA amounts.

Note: For superior first-strand cDNA synthesis performance in two-step RT-qPCR, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. The master mix provides all cDNA synthesis reaction components in a convenient one-tube master mix format. It elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.

사양

검출 방법qPCR

형식Kit

GC-Rich PCR PerformanceHigh

반응 속도60 min.

기술Reverse Transcription

최적 반응 온도42°C

수량50 reactions

역전사 효소SuperScript III

리보뉴클레아제 H 활성Reduced

배송 조건Dry Ice

용도(애플리케이션)Real Time PCR (qPCR)

최종 제품 유형First-Strand cDNA

반응 수50 Reactions

반응 형식Master Mix

시약 유형Reverse Transcription

크기(최종 제품)Up to 10 kb

시작 물질RNA

Unit SizeEach