Invitrogen™

SuperScript™ VILO™ Master Mix

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript자세히 알아보기

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR assays.

Features of SuperScript VILO Master Mix include:
• Starting material can range up to 2.5 μg of total RNA in a 20 μL cDNA synthesis reaction
• Recombinant ribonuclease inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination
• RT is engineered with reduced RNase H activity and increased thermal stability

For superior cDNA synthesis performance in RT-qPCR applications
SuperScript IV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.

사양

검출 방법qPCR

형식Master Mix

GC-Rich PCR PerformanceHigh

반응 속도60 min.

기술Reverse Transcription

최적 반응 온도42°C

수량50 reactions

역전사 효소SuperScript III

리보뉴클레아제 H 활성Reduced

배송 조건Dry Ice

용도(애플리케이션)Real Time PCR (qPCR)

농도5X

최종 제품 유형First-Strand cDNA

반응 수50 Reactions

반응 형식Master Mix

시약 유형Reverse Transcription

크기(최종 제품)Up to 10 kb

시작 물질RNA

Unit SizeEach